The area is of great importance for a number of species of flora and fauna. Over 70 local species are included into Resolution No 6 of the Standing Committee to the Bern Convention. The vegetation of the park includes 857 species of vascular plants. 41 plant species of the park are listed in The Red Data Book of Ukraine: the Lady’s slipper (Cypripedium calceolus), the Floating watermoss (Salvinia natans),the Sand jurinea (Jurinea cyanoides), the Waterwheel (Aldrovanda vesiculosa),the Shrubby birch (Betula humilis), and others.

The local wildlife includes 333 vertebrate species: 30 species of fish, 12 — amphibians, 6 — reptiles, 231 species of birds, and 60 species of mammals. It is of key importance as a nesting place for wetland species, as well as a migration corridor.  Nesting here are globally threatened bird species as the Aquatic warbler (Acrocephalus paludicola), the Great snipe (Gallinago media), the Greater spotted eagle (Aquila clanga), and the European roller (Coracias garrulous). Additionally, the area is of great importance for conservation of wetland bird species, such as the Short-eared owl (Asio flammeus), the Bittern (Botaurus stellaris), the Black tern (Chlidonias niger), the Whiskered tern (Chlidonias hybridus), the White-winged tern (Chlidonias leucopterus), the Montagu’s harrier (Circus pygargus), the Corncrake (Crex crex), the Little bittern (Ixobrychus minutus), the Bluethroat (Luscinia svecica), the Ruff (Philomachus pugnax), the Little crake (Porzana parva), the Spotted crake (Porzana porzana), the Terek sandpiper (Xenus cinereus).

The area is inhabited by such mammal species as the European beaver (Castor fiber), the Common otter (Lutra lutra), the Murin des marais (Myotis dasycneme), the European mink (Mustela lutreola). The territory is an exceptionally important habitat of a number of fish species: the European weatherfish (Misgurnus fossilis), the Ziege (Pelecus cultratus), the Spined loach (Cobitis taenia),the European bitterling (Rhodeus sericeus amarus), аs well as of reptiles and amphibians: the European pond turtle (Emys orbicularis), the Crested newt (Triturus cristatus), the Fire-bellied toad (Bombina bombina). Rare insect species as follow are identified for the area: Dytiscus latissimus, the Scarce fritillary (Hypodryas maturna), the Large copper (Lycaena dispar).

The forest habitats and species are threatened by clearcut logging, sanitation felling, deadwood withdrawal, and forest plantation. The wetland ecosystems are degrading due to polder systems existing both in the national park and in its outskirts. The meadow ecosystems are overgrowing with shrubs due to abandoning of hay-making and grazing, as well as threatened by their transformation into arable lands. The entire national park is negatively impacted by household waste pollution, illegal hunting and fishing.

Сameratraps were installed to study current distribution and abundance of big mammals in the project region.

Equipment, like GPS, office equipment, weather stations, etc. was purchased for the national park.

Since the beginning of the war, funds were raised for the support of Polesian PAs that have been directly affected by the war or need support in accommodation Internally Displaced People. Pripyat-Stokhid National Park was receiving regular financial support for covering expenditures like fuel, vehicle spares and maintenance, building maintenance, services (rent, telecommunications, etc.) and office supplies.